When we ligate a foreign DNA at the sal I site of tetracycline resistance gene in a vector pBR 322, the ____a____ plasmid will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from ____b____ ones by plating the ___c____ on ampicillin containing medium
a b c
1. Non-recombinant Recombinant Transformant
2. Recombinant Non-recombinant Transformant
3. Transformant Non-recombinant Recombinant
4. Recombinant Transformant Non-recombinant
If Pvu I restriction endonuclease is used for cleaving pBR322 cloning vector for inserting foreign DNA, which of the following can be expected in recombinants?
1. They remain resistant to tetracycline but not to ampicillin
2. They would die in tetracycline containing medium but remain alive in ampicillin containing medium
3. Replication of recombinant plasmid get prevented
4. They do not show resistance to ampicillin as well as tetracycline
If the gene of interest is cloned at ECoRI in pBR322,
the recombinant E-coli after transformation are
1. Susceptible to ampicillin and tetracyline.
2. Sensitive to tetracycline.
3. Resistant to kanamycin.
4. Resistant to ampicillin and tetracycline
Which of the following enzyme is involved in the
isolation of genetic material like DNA from a plant cell?
I. Cellulase II. Lysozyme
III. Ribonuclease IV. Protease
1. I, II, III and IV
2. I and II only
3. I only
4. I, III and IV
Similarity between microinjection and biolistic gene
gun method is
1. Used to transform the plant host cells.
2. Used to transform the animal host cells.
3. Recombinant DNA is directly injected into
host cells.
4. Used to transform the bacterial host cells
The product has to be subjected through a series of processes before it is ready for marketing as a finished product called down stream processing. It includes
1. Separation
2. Purification
3. Quality control
4. Both 1 & 2
Match the following enzymes with their functions:
Column-I |
Column-II |
||
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
Options: | (a) | (b) | (c) | (d) |
1. | (iii) | (i) | (iv) | (ii) |
2. | (iii) | (iv) | (i) | (ii) |
3. | (iv) | (iii) | (i) | (ii) |
4. | (ii) | (iv) | (i) | (iii) |
The two antibiotic resistance genes on vector pBR 322 are for:
1. Ampicillin and Tetracycline
2. Ampicillin and Chloramphenicol
3. Chloramphenicol and Tetracycline
4. Tetracycline and Kanamycin
A selectable marker is used to:
1. | help in eliminating the non-transformants so that the transformants can be regenerated. |
2. | identify the gene for the desired trait in an alien organism. |
3. | select a suitable vector for transformation in a specific crop. |
4. | mark a gene on a chromosome for isolation using a restriction enzyme. |
Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
(a) | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
(b) | DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA. |
(c) | The smaller the DNA fragment, the greater the distance it travels through it. |
(d) | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
1. | (a), (c) and (d) | 2. | (a), (b) and (c) |
3. | (b), (c) and (d) | 4. | (a), (b) and (d) |