Q. No.Identify the incorrect statement regarding restriction endonucleases:
1.
They mostly cut dsDNA at specific base sequences.
2.
They are produced by bacterial cells as a mechanism of self-defence.
3.
They digest DNA by removing nucleotides from a free 3' end.
4.
They often generate short single-stranded sequences at the ends of the resultant fragments.
The Ti plasmid, used in transformation of plant cells, is found in:
| 1. | Agrobacterium tumefaciens | 2. | Escherichia coli |
| 3. | Bacillus thuringiensis | 4. | Xanthomonas citri |
Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
Bioprocessing engineering deals with:
| 1. | the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained. |
| 2. | developing methods and software tools for understanding biological data. |
| 3. | production of active pharmaceutical substances in genetically modified organisms (GMOs). |
| 4. | maintenance of sterile ambience in chemical engineering processes. |
Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
The steps included in a cycle of Polymerase Chain Reaction are:
| 1. | initiation, elongation, and termination |
| 2. | transfection, transformation, and transduction |
| 3. | denaturation, annealing, and extension |
| 4. | restriction, electrophoresis, and elution |
Consider the two statements:
| I: | It is desirable to use restriction enzymes produce 'sticky ends' for cloning experiments in genetic engineering. |
| II: | If the donor DNA and the vector DNA are cut by such same restriction endonuclease, ligation of the two is easy. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | Only I is correct |
| 4. | Both I and II are incorrect |
Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:
| 1. | Blue colonies |
| 2. | White colonies |
| 3. | No colonies |
| 4. | Orange colonies if seen through UV light |
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