Q. No.Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
| 1. | Laboratory flask of the largest capacity |
| 2. | A stirred-tank bioreactor without in-lets and out-lets |
| 3. | A continuous culture system |
| 4. | Any of the above |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoRI | 2. | HindII |
| 3. | SmaI | 4. | BamHI |
The following palindrome is recognized by the restriction enzyme:
| 1. | BamHI | 2. | EcoRI |
| 3. | HindII | 4. | PstI |
A method used only for transforming animal cells is:
| 1. | Biolistics | 2. | Microinjection |
| 3. | Use of virus | 4. | Agrobacterium mediation |
Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:
| 1. | Recombinant protein | 2. | Native protein |
| 3. | Pro-protein | 4. | Exotic protein |
Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
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