The European Federation of Biotechnology [EFB] defines biotechnology as:
1. The use of living cells and bacteria in industrial and scientific processes.
2. The integration of natural sciences and organisms, cells, parts thereof, and molecular analogues for products and services.
3. The use of biology to solve problems and make useful products.
4. The use of biology to develop new products, methods and organisms intended to improve human health and society.
Subtopic:  Introduction & History: I |
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Bioprocessing engineering deals with:

1. the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained.
2. developing methods and software tools for understanding biological data.
3. production of active pharmaceutical substances in genetically modified organisms (GMOs).
4. maintenance of sterile ambience in chemical engineering processes.
Subtopic:  Introduction & History: I |
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What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?

I: Inclusion and multiplication of undesirable genes
II: Inability of inclusion of desirable traits from other species
 
1. Only I 2. Only II
3. Both I and II 4. Neither I nor II
Subtopic:  Selectable Markers | Tools |
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:

1. It includes the centromere
2. It becomes a part of chromosome
3. It does not contain any intron sequences
4. It is a single stranded polynucleotide

Subtopic:  Cloning Vector |
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For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:

1. Telomeric sequence 2. Multiple cloning sites
3. Ori 4. Selectable marker

Subtopic:  Cloning Vector |
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?

1. Banting and Best 2. Bolivar and Rodriguez
3. Hershey and Chase 4. Cohen and Boyer
Subtopic:  Restriction Enzymes - Main Enzymes |
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In recombinant DNA experiments, a vector:

1. carries DNA into a new cell 
2. links together newly joined fragments of DN
3. makes millions of copies of a specific segment of DNA
4. separates fragments of DNA by their length and electrical charges
Subtopic:  Cloning Vector |
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The palindrome in DNA is a sequence of base pairs:
1. that reads same on the two strands when orientation of reading is kept the same.
2. that reads same on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other.
3. that are complementary on the two strands when orientation of reading is kept the same. 
4. that are complementary on the two strands when orientation of reading is 5’ to 3’ on one end and 3’ to 5’ on the other.
Subtopic:  Restriction Enzymes - Main Enzymes |
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Identify the incorrect statement regarding restriction endonucleases:

1. They mostly cut dsDNA at specific base sequences.
2. They are produced by bacterial cells as a mechanism of self-defence.
3. They digest DNA by removing nucleotides from a free 3' end.
4. They often generate short single-stranded sequences at the ends of the resultant fragments.
Subtopic:  Restriction Enzymes - Main Enzymes |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:

1.  EcoRI 2.  HindII 
3.  SmaI 4.  BamHI
Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
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