Which of the following is not required in the preparation of a recombinant DNA molecule?
1. Restriction endonuclease                   
2. DNA ligase
3. DNA fragments                                   
4. E. coli

Subtopic:  General Design of an rDNA experiment |
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The role of DNA ligase in the construction of a recombinant DNA molecule is

1. formation of phosphodiester bond between two DNA fragments
2. formation of hydrogen bonds between sticky ends of DNA fragments
3. ligation of all purine and pyrimidine bases
4. None of the above


 

Subtopic:  General Design of an rDNA experiment |
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A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
 

1. human genes may have intron which bacteria cannot process
2. amino acid codons for humans and bacteria are different
3. human protein is formed but degraded by bacteria
4. All of the above


Subtopic:  General Design of an rDNA experiment |
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