The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
1. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
2. The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
3. Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.
4. The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.
Subtopic:  Cloning Vector |
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic:  Cloning Vector |
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Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic:  Cloning Vector |
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
Subtopic:  Cloning Vector |
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 Match List - I with List - II
List-I List-II
(A) Kanamycin (I) Delivers genes into animal cells
(B) ClaI (II) Selectable marker
(C) Disarmed retroviruses (III) Restriction site
(D) Kanamycin Rgene (IV) Antibiotic resistance

Choose the correct answer from the options given below:
Options: (A) (B) (C) (D)
1. (II) (III) (I) (IV)
2. (III) (I) (IV) (II)
3. (IV) (III) (I) (II)
4. (II) (IV) (I) (III)
Subtopic:  Cloning Vector |
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Which  of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic:  Cloning Vector |
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Identify the properties of a good vector used in rDNA technology:

(a) It should have origin of replication supporting a high copy number 
(b) It should have preferably more than '2' recognition sites
(c) The restriction sites in vector should be in the antibiotic- resistant genes 
(d) It should have suitable marker genes 
(e) It should be easy to isolate and purify 


Choose the most appropriate answer from the options given below: 

1. (a), (c) and (e) only  2. (c), (d) and (e) only 
3. (a), (b) and (c) only  4. (a), (c), (d) and (e) only 
Subtopic:  Cloning Vector | Tools |
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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
Subtopic:  Cloning Vector |
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Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain,

1. it will lead to the lysis of host cells.
2. it will be able to produce a novel protein with dual abilities. 
3. it will not be able to confer ampicillin resistance to the host cell. 
4. the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside.

Subtopic:  Cloning Vector |
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The two antibiotic resistance genes on vector pBR 322 are for:

1. Ampicillin and Tetracycline

2. Ampicillin and Chloramphenicol

3. Chloramphenicol and Tetracycline

4. Tetracycline and Kanamycin

Subtopic:  Cloning Vector |
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