DNA fragments digested by restriction endonucleases separate on agar gel electrophoresis according to their size because:
1. | they are forced to move towards cathode under an electric field |
2. | agar gel provides a sieving effect |
3. | larger fragments will have more negative charge and will move farther in the electric field |
4. | chelation with ethidium bromide reduce the rate of transport of fragments |
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