- 1(current)
Q. No.A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
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NEET MCQ Books for XIth & XIIth Physics, Chemistry & Biology1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
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NEET MCQ Books for XIth & XIIth Physics, Chemistry & Biology| Assertion (A): | Polymerase chain reaction is used in DNA amplification. |
| Reason (R): | The ampicillin resistant gene is used as a selectable marker to check transformation. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is not correct but (R) is correct. |
| 2. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
| 3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
| 4. | (A) is correct but (R) is not correct. |
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NEET MCQ Books for XIth & XIIth Physics, Chemistry & BiologyIn a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
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NEET MCQ Books for XIth & XIIth Physics, Chemistry & BiologyDuring the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
| 1. | Denaturation | 2. | Ligation |
| 3. | Annealing | 4. | Extension |
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NEET MCQ Books for XIth & XIIth Physics, Chemistry & BiologyMatch the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
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NEET MCQ Books for XIth & XIIth Physics, Chemistry & Biology- 1(current)
Q. No.
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