Assertion(A): | Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with roots of pulse crops. |
Reason(R): | A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated |
In the following questions a statement of assertion (A) is followed by a statement of reason (R).
1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
3. | (A) is True but (R) is False. |
4. | Both (A) and (R) are False. |
Statement I: | Proteins encoded by genes cryIAc and cryllAb control corn borer, that of crylAb control the cotton bollworms |
Statement II: | Bt toxin protein exist as inactive protoxins but once an insect ingests the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut |
1. | Human protein –1–antitrypsin. |
2. | Human alpha–lactalbumin. |
3. | Human insulin–like growth factor. |
4. | Human enzyme Adenosine Deaminase (ADA). |
1. | Secretin | 2. | Insulin |
3. | Myoglobin | 4. | Urease |
1. | Gene therapy – Use of retrovirus to introduce cDNA into the host cell. |
2. | PCR – Used to detect mutations is genes in suspected cancer patients |
3. | ELISA – Detection of non-proteinecious antigens. |
4. | Probe – A single strand DNA or RNA, tagged with radioactive molecule, will never hybridized with mutated gene |
(i) | PCR is used to detect mutations in genes in suspected cancer patients. |
(ii) | A direct method to insert a foreign gene into plant cells is micro-injection. |
(iii) | The first transgenic cow, Rosie, produced human protein-enriched milk, i.e., 5.4 grams per litre. |
(iv) | RNAi takes place in all eukaryotic organisms as a method of cellular defense. |
Column I | Column II | ||
a. | Patent on Basmati rice by an American company | (i) | 1983 |
b. | First clinical gene therapy | (ii) | 1997 |
c. | Humulin by Eli Lilly Co. | (iii) | 1972 |
d. | Construction of first recombinant DNA | (iv) | 1990 |