What is true A,B and C in the given diagrammatic representation of rDNA technology ?
I. At A same restriction enzyme is used to cut both foreign and vector DNA
II. The enzyme used at B is DNA ligase
III. Step C can be called as transformation
1. I and II only
2. I and III only
3. II and III only
4. I,II and III
The process of separation and purification of expressed protein before marketing is called
(1) upstream processing
(2) downstream processing
(3) bioprocessing
(4) postproduction processing
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
(1) The larger the fragment size, the farther it moves
(2) The smaller the fragment size, the farther it moves
(3) Positively charged fragments move to farther end
(4) Negatively charged fragments do not move
A gene, whose expression helps to identify transformed cells is known as
1. selectable marker
2. vector
3. plasmid
4. structural gene
The DNA fragments separated on an agarose gel can be visualised after staining with
(1) bromophenol blue
(2) acetocarmine
(3) aniline blue
(4) ethidium bromide
Stirred-tank bioreactors have been designed for:
1. purification of product
2. addition of preservatives to the product
3. availability of oxygen throughout the process
4. ensuring anaerobic conditions in the culture vessel
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
(1) Eco RI
(2) taq polymerase
(3) polymerase III
(4) ligase
Which of the following is not a component of downstream processing?
1. Separation
2. Purification
3. Preservation
4. Expression
Which of the following restriction enzymes produces blunt ends?
(1) Sal I
(2) Eco RV
(3) Xho
(4) Hind III
Which of the following is a endonuclease?
(1) Protease
(2) DNase I
(3) RNase restriction
(4) Hind II