The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
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The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

Subtopic:  Large Scale Production: I | Large Scale Production |
 59%
From NCERT
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The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Subtopic:  Large Scale Production: I | Large Scale Production |
 54%
From NCERT
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Which of the following doesn't go with PCR?

A.      Thermus aquaticus

B.      1 billion copies

C.      Taq polymerase

D.     Dideoxynucleotides

Subtopic:  Large Scale Production: I | Large Scale Production |
 89%
From NCERT
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What is the final step of PCR?

A.      Extension

B.      Annealing

C.      Denaturation

D.     Extraction

Subtopic:  Large Scale Production: I | Large Scale Production |
 85%
From NCERT
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After 30 cycle of PCR, how many times DNA will be amplified?

A.      Thousand times

B.      Million times

C.      Billion times

D.     Hundreds times

Subtopic:  Large Scale Production: I | Large Scale Production |
 87%
From NCERT
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Use of primers, occurs in which step of PCR?

A.      Annealing

B.      Extension

C.      Denaturation

D.     Both extension and Annealing

Subtopic:  Large Scale Production: I | Large Scale Production |
 77%
From NCERT
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If you have transformed cell with recombinant DNA and it grows on kanamycin and chloramphenicol containing plates but does not grow on ampicillin containing medium. What does it imply?

A.      The cell has resistance against ampicillin but not against chloramphenicol and kanamycin

B.      Has resistance against both kanamycin and chloramphenicol but not against ampicillin

C.      We may not confer anything from it

D.     It depends upon gene of interest

Subtopic:  Host & Desired DNA: I | Host & Desired DNA |
 74%
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The ultimate aim of maximum recombinant DNA technologies is

1. To multiply DNA

2. To express DNA

3. To multiply mRNA

4. To express protein

Subtopic:  Large Scale Production: III | Large Scale Production |
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Which of the following processes is not associated with protein produced by recombinant DNA?

A.      Restriction Digestion

B.      Expression

C.      Optimization

D.     Purification

Subtopic:  Large Scale Production |
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