If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

A.      Cellulase

B.      Chitinase

C.      Lysozyme

D.     Protease

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
From NCERT
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If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?

A.      DNAse

B.      Ribonuclease

C.      Protease

D.     Lysozyme

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 62%
From NCERT
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The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 97%
From NCERT
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The end result of precipitation of DNA in suspension is seen

A.      In the form of dark solution

B.      In the form of effervescence

C.      In the form of fine threads

D.     In the form of bubbles

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
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The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
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The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

Subtopic:  Large Scale Production: I | Large Scale Production |
 59%
From NCERT
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The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Subtopic:  Large Scale Production: I | Large Scale Production |
 54%
From NCERT
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Which of the following doesn't go with PCR?

A.      Thermus aquaticus

B.      1 billion copies

C.      Taq polymerase

D.     Dideoxynucleotides

Subtopic:  Large Scale Production: I | Large Scale Production |
 89%
From NCERT
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What is the final step of PCR?

A.      Extension

B.      Annealing

C.      Denaturation

D.     Extraction

Subtopic:  Large Scale Production: I | Large Scale Production |
 85%
From NCERT
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After 30 cycle of PCR, how many times DNA will be amplified?

A.      Thousand times

B.      Million times

C.      Billion times

D.     Hundreds times

Subtopic:  Large Scale Production: I | Large Scale Production |
 87%
From NCERT
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