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Identify the gene and its protein that controls corn borer from the given options
(1) cry I Ab and Cry I Ab
(2) cry I Ac and Cry I Ac
(3) cry II Ab and Cry II Ab
(4) cry I Ac and Cry I Ac

Bt toxin kills the insect by
(1) Blocking mitochondrial respiration
(2) Blocking transfer of nerve impulse
(3) Creating pores in body surface
(4) Creating pores in mid-gut epithelial cell

The bacterium Bacillus thuringiensis is widely used in contemporary biology as
(1) Bioweapon
(2) Bioinsecticide
(3) Bioweedicide
(4) Indicator of water pollution

How many recombinant therapeutics have been approved for human use in world over?
(1) 12
(2) 30
(3) 42
(4) 27

Why would lac z gene be fused to coding sequence of chain A and B in plasmids of E.coli?
(1) Helps to form blue insulin which is more effective
(2) Helps in selection of recombinant
(3) Help in oral administration of insulin
(4) All of these

Which enzyme was targetted during the first clinical gene therapy given in 1990 to a four year- old girl?
(1) Monoamine oxidase
(2) Tyrosine oxidase
(3) Pyruvate dehydrogenase
(4) Adenosine deaminase

If a probe is made of radioactively labelled dsDNA and is allowed to hybridise to its complementary DNA in a cloned cell followed by detection using autoradiography. Which of the following is considered wrong for the given statements?
(1) It is double stranded DNA
(2) It is radioactively labelled
(3) This probe can be used in autoradiography
(4) It is hybridise to complementary DNA/RNA

Techniques that serve the purpose of early diagnosis include
(1) PCR
(2) ELISA
(3) Serum analysis
(4) Both (1) & (2)

Which of the following protein product has been used to treat emphysema?
(1) $\alpha$-lactalbumin
(2) TPA
(3) $\alpha$-1-antitrypsin
(4) C-peptide

‘Rosie’ a transgenic cow is known to produce a type of milk which has all the following charcteristics, except
(1) Protein content of 2.4 g/L
(2) Human $\alpha$-lactalbumin
(3) More balanced diet than normal cow milk for babies
(4) Was produced for first time in year 2001

According to the latest estimates how many documented varieties of basmati rice are grown in India?
(1) 30
(2) 27
(3) 118
(4) 42

Which of the Indian plants have either been patented or attempts have been made to patent them by western nations for their commercial use?
(1) Daffodil
(2) Neem
(3) Turmeric
(4) Both (2) & (3)

Which of the following is a transgenic plant?
(1) Flavr savr
(2) Meloidogyne incognita
(3) Caenorhabditis elegans
(4) Oryza sativa

Select incorrect statement with respect to RNAi
(1) dsDNA binds target mRNA and initiate RNAi
(2) Agrobacterium vector is used to introduce nematode specific gene into host plant
(3) ssRNA binds target mRNA and initiate RNAi
(4) Both (2) & (3)

Infestation by tobacco budworm and armyworm is prevented by Bt toxin. These insects belong to order
(1) Coleoptera
(2) Lepidoptera
(3) Diptera
(4) Hymenoptera

Select the incorrect match
(1) cry I Ab - Corn borer
(2) Bt toxin - Beetles
(3) cry I Ac - Cotton Bollworm
(4) cryIIAc - Cotton Bollworm

All the following statements are correct for Bt toxin but one is wrong. Which one is wrong?
(1) Bt toxin is produced by bacteria called Bacillus thuringiensis
(2) Bt toxin are protein toxins like thurioside and sporeine which are active against different group of insects
(3) Bt toxin is harmful for man
(4) Upon ingestion by susceptible insects the Bt protoxin is converted into active form and kills the insects

Which of the following is correct statement with respect to animal insulin?
(1) It is just as effective as human insulin over prolonged time
(2) It does not elicit immune response ever
(3) It can be orally administrated to diabetic people
(4) It was extracted from pancreas of slaughtered cattle and pigs

Injecting functional adenosine deaminase into a patients (lacking ADA gene) blood cells may be considered as
(1) Gene therapy
(2) Enzyme replacement therapy
(3) Both (1) & (2)
(4) Genetic engineering

Technique used to detect HIV in suspected AIDS patient in asymptomatic stage is
(1) PCR
(2) Serum analysis
(3) Both (1) & (2)
(4) Urine analysis

Eli Lilly an American company is famous for
(1) Producing GH (growth hormone) synthesised by recombinant DNA technology
(2) Preparing two DNA sequences corresponding to A and B chains of human insulin and introduced them in the plasmids of E. coli to produce insulin chains
(3) Producing pest resistant plants, by RNA interference
(4) Producing vitamin ‘A’ enriched rice

The use of bioresources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is called
(1) Bioethics
(2) Biopiracy
(3) Bioterror
(4) Bioweapon

In vitro clonal propagation in plants is characterized by
(1) PCR and RAPD
(2) Northern blotting
(3) Electrophoresis and HPLC
(4) Microscopy

An analysis of chromosomal DNA using the Southern hybridization techinque does not use: 
(1) Electrophoresis
(2) Blotting
(3) Autoradiography
(4) PCR

Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of R-DNA technology.
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host. Which of the two statements having mistakes?
(1) (A) and (C)
(2) (A) and (B)
(3) (B) and (C)
(4) (C) and (D)

Which of the following statements about Bacillus thuringiensis are correct?
A. One of the toxin produced by the bacteria is thurioside, which is active against different groups of insect larvae
B. The toxin accumulates inside the bacteria during sporulation
C. Upon ingestion by susceptible insects they are converted into active form and kill them by inhibition of ion transport in the midgut
D. The proteins encoded by the gene cry II Ab controls corn borer
(1) B only
(2) A and B
(3) A, B and C
(4) A, B, C and D

A regulatory body working under MoEF for the release of transgenic crops is
(1) NBPGR
(2) GEAC
(3) NSC
(4) NIPGR

A : RNAi takes place in all eukaryotic organisms as a method of cellular defense.
R : Complementary dsRNA molecule binds to specific mRNA and prevents its translation (silencing).

A : Recombinant DNA technologies process has been less effective in therapeutic drug production.
R : Recombinant therapeutics induce unwanted immunological responses.

A : ELISA can enable very easy detection of infection through antigen antibody interaction.
R : It is a nucleic acid based diagonostic tool that can confirm presence of infectious microbe at early stages.

A : Production of hirudin from transgenic Brassica required use of synthetic gene.
R : Eukaryotic genes have intron sequences that need to be spliced out.