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Methods of producing microbe and pest resistant plants include
(1) RNAi
(2) Use of Bt toxin
(3) Gene therapy
(4) Both (1) & (2)

Which of the following is not an advantage of GM crops?
(1) Increased crop tolerance to salt in soil
(2) Increased reliance on chemical pesticides
(3) Reduction in post harvest losses
(4) Increased efficiency of mineral usage

Which of the following may be considered as an application of biotechnology?
(1) Bioremediation
(2) Waste treatment
(3) Energy production
(4) All of these

Hirudin can be extracted from transgenic plant
(1) Brassica napus
(2) Bacillus napus
(3) Bt brinjal
(4) Bt Brassica napus

Main objective of production/use of herbicide resistant GM crops is to 
(A) - Reduce herbicide accumulation in food articles for health safety.
(B) - Eliminate weeds in the field without the use of herbicides.
(C) - Eliminate weeds from the field without the use of manual labour.
(D) - Soil pollution and biomagnification caused by herbicides.
Which of the following statements are correct?
(1) (A) only
(2) (B) only
(3) (A), (B),(C) & (D)
(4) (C) only

A doctor while operating on HIV positive patient accidently cuts himself with a scalpel.  In order to confirm whether he has contracted the virus which of the following tests should be performed?
(1) Serum analysis 
(2) Urine analysis
(3) Blood analysis
(4) PCR

Disadvantage of using porcine insulin in diabetic patients is 
(1) That it may lead to hypercalcemia
(2) It may cause allergic reactions
(3) It is expensive
(4) It can lead to mutations in adult

Enzyme used in ELISA test is 
(1) Endonuclease
(2) Ligase
(3) Peroxidase
(4) Polymerase

A probe is used in which stage of genetic engineering?
(1) Cleaving DNA
(2) Recombining DNA
(3) Cloning
(4) Screening

Which of the following genetically engineered microbe is used in scavening of oil spills by digesting hydrocarbons of crude oil?
(1) Pseudomonas fluorescence
(2) Rhizobium meliloti
(3) Pseudomonas putida
(4) Trichoderma

What could be the possible harm of using antibiotic resistant gene as a selectable marker in plasmid, used for gene transfer in production of GM food?
(A) GM food contains the enzymes produced by the antibiotic resistance gene that was used during gene transfer in genetic engineering.  This could cause allergies since it is a foreign protein.
(B) The bacteria present in the alimentary canal of the humans could take up the antibiotic resistance gene present in GM food.
(C) The bacteria in the human alimentary canal would then become resistant to the concerned antibiotic.
(D) The transgene may be transferred through pollen to their wild relatives and make weeds more persistant and damaging.
(1) (A) only
(2) Both (B) & (C)
(3) (A), (B) & (C)
(4) (A), (B), (C) & (D)

What is the difference between the two mice as shown as in the figure?

(1) One is normal in size, the other is twice as big supermouse because of good diet.
(2) The bigger 'Supermouse' is transgenic.  It is larger because of expression of the gene for human growth hormone factor that has been introduced.
(3) The smaller one is a dwarf
(4) Transgenic mice are an example of gene therapy

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
(1) Retrovirus
(2) Ti plastid
(3) pBR 322
(4) $\lambda$ phage

Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
(1) Bio-infringement
(2) Biopiracy
(3) Bioexploitation
(4) Biodegradation

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is
(1) Indian Council of Medical Research(ICMR)
(2) Council for Scientific and Industrial Research(CSIR)
(3) Genetic Engineering Appraisal Committee(GEAC)
(4) Research Committee on Genetic Manipulation(RCGM)

The process of separation and purification of expressed protein before marketing is called
(1) Upstream processing
(2) Downstream processing
(3) Bioprocessing
(4)Postproduction processing

Which kind of therapy was given in 1990 to a four- year-old girl with adenosine deaminase (ADA) deficiency?
(1) Gene therapy
(2) Chemotherapy
(3) Immunotherapy
(4) Radiation therapy

The two polypeptides of human insulin are linked together by
(1) Disulphide bridges
(2) Hydrogen bonds
(3) Phosphodiester bond
(4) Covalent bond

Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of
(1) Vitamin A
(2) Vitamin B
(3) Vitamin C
(4) Omega 3

The introduction of t-DNA into plant involves
(1) Allowing the plant roots to stand in water 
(2) Infection of the plant by Agrobacterium tumefaciens
(3) Altering the pH of the soil, then heat-shocking the plants
(4) Exposing the plants to cold for a brief period

In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to
(1) Presence of conversion factors in insect gut
(2) Alkaline pH of the insect gut
(3) Acidic pH of the insect gut
(4) Action of gutmicro-organisms

In vitro clonal propagation in plants is characterized by
(1) PCR and RAPD
(2) Northern blotting
(3) Electrophoresis and HPLC
(4) Microscopy

Commonly used vectors for human genome sequencing are
(1) T-DNA
(2) BAC and YAC
(3) Expression vectors
(4) T / A cloning vectors

The first human hormone produced by recombinant DNA technology is
(1) Insulin
(2) Estrogen
(3) Thyroxin
(4) Progesterone

A single strand of nucleic acid tagged with a radioactive molecule is called
(1) Plasmid
(2) Probe
(3) Vector
(4) Selectable marker

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
(1) Both sense and anti-sense RNA
(2) A particular hormone
(3) An antifeedant
(4) A toxic protein

Maximum number of existing transgenic animals is of
(1) Pig
(2) Fish
(3) Mice
(4) Cow

Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of R-DNA technology.
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host.
Which of the two statements having mistakes?
(1) (A)and(C)
(2) (A)and(B)
(3) (B)and(C)
(4)(C) and(D)

Bacillus thuringiensis forms protein crystals which contain insecticidal protein.This protein:
(1) Is activated by acid pH of the foregut of the insect pest
(2) Does not kill the carrier bacterium which is itself resistant to this toxin
(3) Binds with epithelial cells of midgut of the insect pest ultimately killing it
(4) Is coded by several genes including the gene cry

Silencing of mRNA has been used in producing transgenic plants resistant to
(1) White rusts
(2) Bacterial blights
(3) Bollwroms
(4) Nematodes

The genetically-modified (GM) brinjal in India has been developed for
(1) Drought-resistance
(2) Insect-resistance
(3) Enhancing shelf life
(4) Enhancing mineral content

Genetic engineering has been successfully used for producing
(1) Animals like bulls for farm work as they have super power
(2) Transgenic mice for testing safety of polio vaccine before use in humans
(3) Transgenic models for studying new treatments for certain cardiac diseases
(4) Transgenic Cow-Rosie which produces high fat milk for making ghee

Polyethylene glycol method is used for
(1) Biodiesel production
(2) Seedless fruit production
(3) Energy production from sewage
(4) Gene transfer without a vector

Transgenic plants are the ones
(1) Generated by introducing foreign DNA into a cell and regenerating a plant from that cell.
(2) Produced after protoplast fusion in artificial medium.
(3) Grown in artificial medium after hybridization in the field.
(4) Produced by a somatic embryo in artificial medium.

The genetic defect – adenosine deaminase (ADA) deficiency may be cured permanently by
(1) Administering adenosine deaminase activators.
(2) Introducing bone marrow cells producing ADA into cells at early embryonic stages.
(3) Enzyme replacement therapy.
(4) Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA

Cry I endotoxins obtained from Bacillus thuringiensis are effective against
(1) Boll worms
(2) Mosquitoes
(3) Flies
(4) Nematodes

What is antisense technology?
(1) RNA polymerase producing DNA
(2) A cell displaying a foreign antigen used for synthesis of antigens
(3) Production of somaclonal variants in tissue cultures
(4) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of
(1) Bacillus 
(2) Pseudomonas 
(3) Trichoderma
(4) Xanthomonas

Which of the following statements about Bacillus thuringiensis are correct?
(A) One of the toxin produced by the bacteria is thurioside, which is active against different groups of insect larvae
(B) The toxin accumulates inside the bacteria during sporulation
(C) Upon ingestion by susceptible insects they are converted into active form and kill them by inhibition of ion transport in the midgut
(D) The proteins encoded by the gene cry II Ab controls corn borer
(1) B only
(2) A and B
(3) A, B and C
(4) A, B, C and D

Which of the following is used as a bioweapon?
(1) Bacillus subtilis
(2) Bacillus licheniformis
(3) Bacillus thuringiensis
(4) Bacillus anthracis

ANDi is a transgenic
(1) Plant
(2) Goat
(3) Monkey
(4) Dog

Why is usually insulin not administered orally to a diabetic patient?
(1) Insulin is bitter in taste
(2) Insulin is a peptide
(3) Insulin will lead to a sudden decrease in blood sugar if given orally
(4) Insulin leads to peptic ulcer if taken orally

Which of the following techniques serve the purpose of early diagnosis than most conventional methods of diagnosis?
(A) Recombinant DNA technology
(B) PCR
(C) ELISA
(1) A only
(2) A & C only
(3) A & B only
(4)A, B & C

Choose the incorrect statement with respect to Ethical issues in biotechnology
(1) Basmati rice is distinct for its unique aroma and flavour and 27 documented varieties of Basmati are grown in India
(2) In 1997, an American company got patent rights on Basmati rice through the US Patent and Trademark office.
(3) Biopiracy is the form used to refer to the use of bioresources by multinational companies without proper authorisation from the countries and people concerned without compensatory payment
(4) The current interest in the manipulation of microbes, plants and animals has raised no ethical questions.

Some of the steps involved in the production of humulin are given below. Choose the correct sequence
(i) Synthesis of gene (DNA) for human insulin artificially.
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of synthetic human insulin gene into plasmid
(v) Introduction of recombinant plasmid into E.coli
(vi) Extraction of recombinant gene product from E.coli
(1) ii, i, iv, iii, v, vi
(2) i, iii, v, vi, ii, iv
(3) i, iv, v, ii, vi, iii
(4) iii, v, ii, i, vi, iv